WebRestriction site coordinates refer to the position of the 5´-most base on the top strand in each recognition sequence. Open reading frame (ORF) coordinates are in the form ... BsrGI, BstBI, BstEII, BstYI, BstZ17I, Bsu36I, ClaI, DpnI, DpnII, EagI, EcoNI, EcoO109I, EcoRI, EcoRV, FauI, FseI, FspAI(x), HindIII, WebApr 7, 2016 · A DNA smear on an agarose gel after a restriction digest can result from one, or more, of the following: 1. nuclease contamination in the digest reaction. 2. issues with the running buffer in the gel box or. 3. the restriction enzyme has a high binding afinity to the DNA. The source of nuclease contamination may come from the DNA preparation ...
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WebDec 15, 2024 · BsrGI. This product was discontinued on 12/15/2024 and is replaced with the high fidelity version, BsrGI-HF™ ( NEB #R3575 ). We are excited to announce that … WebWe are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing … sigma art lens 24mm
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WebJun 12, 2024 · This analysis demonstrated that the ΔGT mutation within the NCF1 gene and its pseudogenes can be corrected, and that two out of three tested sgRNAs (sgRNA #1 and #2; Figures 1B and 1C) successfully reconstituted the BsrG1 restriction site (Figure 1B), restoring the normal NCF1 gene sequence. Interestingly, CRISPR-Cas9 treatment of … WebThermo Scientific PfoI restriction enzyme recognizes T^CCNGGA sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. WebApr 12, 2024 · The WW domain deletion construct was generated by obtaining a synthesized gene block from integrated DNA technologies (IDTs) of a partial WAC coding domain that lacked the WW DNA sequence (encoding amino acids 133–165) with flanking 5′ BsrGI and internal 3′ NsiI restriction sites that were used to ligate in the GFP-WAC … sigma ats pure 1