Chd8 antibody
WebAntibody was affinity purified using an epitope specific to CHD8 immobilized on solid support. The epitope recognized by A301-224A maps to a region between residue 325 and 350 of human chromodomain helicase DNA binding protein 8 using the numbering given in entry NP_065971.2 (GeneID 57680). Immunoglobulin concentration was determined by ... WebSep 7, 2016 · Anti-CHD8 L monoclonal antibody was generated by the lymph-node method 32 with the use of a fusion protein of mouse CHD8 L and glutathione S-transferase (GST) synthesized in Escherichia coli ...
Chd8 antibody
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WebFeb 21, 2024 · RIP was carried out using CHD8 antibody and the IgG antibody, which was served as negative control . Briefly, the cells were harvested by RIPA lysis buffer containing Protease Inhibitor Cocktail and RiboLock RNase inhibitor (1%, v/v) (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacture’s instruction. Obtained … WebJun 5, 2024 · This is a Validated Antibody Database (VAD) review about human CHD8, based on 10 published articles (read how Labome selects the articles), using CHD8 antibody in all methods.It is aimed to help Labome visitors find the most suited CHD8 antibody. Please note the number of articles fluctuates since newly identified citations …
WebThe product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB ... WebImmunoprecipitation - Anti-CHD8 antibody (ab224830) CHD8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T …
WebThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies. All characterization data for ENSG00000100888 on the Human … WebApr 8, 2024 · In small-cell lung cancer (SCLC), the mutation in APC and CHD8, which inhibits transcription mediated by CTNNB1, is related to the relapse of SCLC . In non-small cell lung cancer (NSCLC), Wnt ligands (Wnt1, Wnt2, ... Katoh, M. Antibody-drug conjugate targeting protein tyrosine kinase 7, a receptor tyrosine kinase-like molecule involved in …
WebATP dependent helicase CHD8 antibody; ATP-dependent helicase CHD8 antibody; Axis duplication inhibitor antibody; Beta catenin binding protein like antibody; CHD 8 antibody; …
WebReferences Antibody type Host Reactivity Conjugate Antigen sequence Epitope Applications Western blot ELISA Immunocytochemistry Immunoprecipitation … shuttle robot arm observed from the deckWebJul 22, 2024 · CHD8 was first identified as a negative regulator of the Wnt/β-catenin signaling pathway and is one of the most frequently mutated genes involved in the … shuttle river walkWebDec 27, 2024 · For immunoprecipitation (IP), 1.5 µg anti-CHD8 or anti-HA antibody was added to ChIP buffer (RIPA buffer supplemented with protease inhibitors, PMSF, and 5 mM EDTA) left to rotate O/N at 4 °C. ... shuttle robotWebAntibodies that detect CHD8 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry and ChIP. These antibodies target CHD8 in Human, Mouse and Rat samples. Our … shuttle rexburg to idaho falls airportWebThe antibodies are designated mAB for monoclonal and pAb for polyclonal. The release of the Human Protein Atlas in which the antibody was first published. All assays through … shuttler footballWebBethyl Laboratories Anti-CHD8 Polyclonal, Catalog # IHC-00246. Tested in Immunohistochemistry (IHC) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (50 µg/mL). shuttle rochester mn to minneapolis airportWebAll lanes : Anti-CHD8 antibody (ab224830) at 0.1 µg/ml Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate ... shuttle rochester mn to msp