Phospho antibody
WebThis protocol describes how to detect phosphorylated proteins by western blotting using Phospho-Specific Validated PrecisionAb Antibodies. We also provide a list of reagents … WebPhospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not cross-react with …
Phospho antibody
Did you know?
WebPhospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This … WebAnti-PD1 (phospho Y248) antibody [EPR19821] See all PD1 primary antibodies Description Rabbit monoclonal [EPR19821] to PD1 (phospho Y248) Host species Rabbit Tested applications Suitable for: WB, Dot blot, …
WebPhospho-specific antibodies detect only the phosphorylated forms of proteins in a complex protein mixture within cells. They recognize the phosphorylated amino acids in the … WebPhospho-S6 (Ser235, Ser236) Antibody (MA5-15140) in ICC/IF. Immunofluorescence analysis of Phospho-S6 (Ser235,Ser236) was performed using 70% confluent log phase U …
WebThe cells are stimulated, fixed, and blocked in the same well. Phospho-specific antibodies are used to assess phosphorylation status using fluorometric (Figure 4) or colorimetric detection systems. Furthermore, the phospho-protein and total protein are simultaneously detected in the same microplate well. WebCheck the product webpage/datasheet for recommended antibody dilution. Note that while the membrane is blocked with nonfat dry milk, phospho-specific antibodies are often diluted and incubated with the membrane in 5% BSA. Use TBS-T rather than PBS-T in wash/incubation buffers since the phosphate ion may lead to interference.*
WebFig. 5 Forskolin treatment enhances serine phosphorylation of PCBP1 as analyzed by immunoprecipitation. A) The upper set of gel images shows the enhanced phosphorylation of PCBP1 with forskolin treatment by …
WebFor those antibodies which are suspected to be more intensely/prominently binding, one can or advised to dilute it in 5% NFM (non-fatty milk) But there are antibodies which are very weakly... church on 75thWebProduct name Anti-Phospho - (Ser/Thr) Phe antibody See all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species … church on 68 greensboro ncWebABL1/2 (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429. Form: Liquid Purification: Immunoaffinity purification Concentration: 1 mg/mL Quality Control Testing: Antibody Reactive Against Synthetic Peptide. Recommend Usage: Immunohistochemistry (1:50-1:100) Immunofluorescence … church on 79th and euclidWebAnti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244) from rabbit, purified by affinity chromatography Synonym (s): 40S ribosomal protein S6, Phosphoprotein NP33 eCl@ss: 32160702 Pricing and availability is not … dewey newton turnerWebThe NeuroMab™ anti-Phospho-NDRG1 (Thr346) antibody detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366. dewey nelson alexander ramseyWebAnti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample Other Materials Required Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors church on 75WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. church on 63rd dorchester in chicago